1.     Water Sample Collection for eDNA: Filtration Method. Yuwei Xie, Abigail DeBofsky, Jenna Zee, Tim Jardine, Markus Hecker and John P. Giesy. Version 2, 4/30/2018.

2.     Water Sample Collection for eDNA: Ethanol Preserved Method. Yuwei Xie, Abigail DeBofsky, Markus Hecker and John P. Giesy. Version 2, 4/30/2018.

3.     Surficial Sediment Collection for eDNA Analysis. Yuwei Xie, Abigail DeBofsky, Markus Hecker and John P. Giesy. Version 2, 4/30/2018.

4.     Periphyton Collection for DNA Metabarcoding. Yuwei Xie, Abigail DeBofsky and John P. Giesy. Version 2, 4/30/2018.

5.     Zooplankton collection for DNA metabarcoding. Yuwei Xie, Abigail DeBofsky and John P. Giesy. Version 2, 4/30/2018.

6.     Water Collection for DNA Metabarcoding of Phytoplankton. Yuwei Xie and John P. Giesy. Version 2, 4/30/2018.

7.     MAINTENANCE OF eDNA SAMPLE: CHAIN-OF-CUSTODY. Yuwei Xie, Paul D. Jones and John P. Giesy. Version 1, 4/30/2018.

8.     Field eDNA Sampling Protocol for Biomonitoring of Remote Sites Using the eDNA Sampling Kit. Yuwei Xie, Abigail DeBofsky, Markus Hecker and John P. Giesy. Version 1, 4/30/2018.

9.     Management of eDNA sample: Receiving, Preservation, Storage, Documentation, Decontamination, and Disposal. Yuwei Xie, Markus Hecker, Paul D. Jones and John P. Giesy. Version 1, 4/30/2018.

10.   Maintenance of eDNA Sample Integrity, and Proper Usage of Refrigerators and Freezers. Yuwei Xie, Markus Hecker, Paul D. Jones and John P. Giesy. Version 1, 4/30/2018.

11.   Field Collection of Freshwater Macroinvertebrates. Yuwei Xie, Paul D. Jones and John P. Giesy. Version 1, 4/30/2018.

12.   Isolating eDNA from Water. Yuwei Xie and John P. Giesy. Version 1, 4/30/2018.

13.   Isolating eDNA from Sediments. Yuwei Xie and John P. Giesy. Version 1, 4/30/2018.

14.   Isolating eRNA from Water. Yuwei Xie and John P. Giesy. Version 1, 4/30/2018.

15.   Isolating eRNA from Sediments. Yuwei Xie and John P. Giesy. Version 1, 4/30/2018.

16.   First strand cDNA synthesis of eRNA. Yuwei Xie and John P. Giesy. Version 1, 4/30/2018.

17.   Metabarcoding of Bacterial Communities. Yuwei Xie and John P. Giesy. Version 1, 4/30/2018.

18.   Metabarcoding of Protistan Communities. Yuwei Xie and John P. Giesy. Version 1, 4/30/2018.

19.   Metabarcoding of Diatom Communities. Yuwei Xie and John P. Giesy. Version 1, 4/30/2018.

20.   Metabarcoding of Zooplankton Communities. Yuwei Xie and John P. Giesy. Version 1, 4/30/2018.

21.   Metabarcoding of macro-invertebrate Communities. Yuwei Xie and John P. Giesy. Version 1, 4/30/2018.

22.   Metabarcoding of Fish Communities. Yuwei Xie and John P. Giesy. Version 1, 4/30/2018.

23.   Metabarcoding of Vertebrate Communities. Yuwei Xie and John P. Giesy. Version 1, 4/30/2018.

24.   Quality-check, cleaning, quantification and pooling of PCR products. Yuwei Xie and John P. Giesy. Version 1, 4/30/2018.

25.   Co-isolation of eDNA/eRNA/eProtein from Water Using Qiagen AllPrep® DNA/RNA/Protein Mini Kit. Yuwei Xie and John P. Giesy.  Version 1, 9/19/2018.

26.   Co-isolation of DNA/RNA/Protein from Fish Tissues Using Qiagen AllPrep® DNA/RNA/Protein Mini Kit. Yuwei Xie and John P. Giesy. Version 1, 9/19/2018.

27.   Co-isolation of eDNA/eRNA from Sediment. Yuwei Xie and John P. Giesy. Version 1, 9/19/2018.

28.   Co-isolation of DNA/RNA/Protein from Cells Using Qiagen AllPrep® DNA/RNA/Protein Mini Kit. Yuwei Xie and John P. Giesy. Version 1, 12/24/18.

29.   Sub-chronic aqueous chemical exposure for assessing gut microbiome perturbations in fishes. Alana Weber, Abigail DeBofsky, Yuwei Xie and John P. Giesy. Version 1, 7/11/19.

30.   Metabarcoding of Cyanobacteria Assembly.Yuwei Xie and John P. Giesy. Version 1, 8/2/19.

31.   Preparation of Sequencing Library for Illumina Platform. Yuwei Xie and John P. Giesy. Version 1, 8/2/19.

32.   Amplicon Next-generation sequencing by use of Illumina Miseq. Yuwei Xie and John P. Giesy. Version 1, 8/2/19.

33.   Amplicon Next-generation sequencing by use of Illumina NextSeq 500 platform. Yuwei Xie and John P. Giesy. Version 1, 8/2/19.

34.   Bioinformatics pipeline for metabarcoding. Yuwei Xie and John P. Giesy. Version 1, 89/2/19.